Post by rabia373 on Mar 11, 2024 22:56:51 GMT -5
Therapy was tested in the laboratory on cells cultured using the Magnetic Levitation Method. Laboratory tests carried out at the Optics Group of the São Carlos Institute of Physics (IFSC) at USP demonstrated that Photodynamic Therapy (PDT), a methodology that consists of the association of three components — light, photosensitizer and molecular oxygen present in the tissue to be treated — could be applied in the treatment of melanoma (skin cancer). Researcher Larissa Satiko Alcântara Sekimoto, a member of the group, developed PDT tests on melanoma cells, cultivated using a three-dimensional culture model (or in vitro tissue ) called the Magnetic Levitation Method and which was created a few years ago in the U.S. In search of a possible treatment via PDT for patients with melanoma, Larissa, who was guided by professor Cristina Kurachi, from IFSC, developed in vitro tests on melanoma tumors, obtained from the aforementioned Magnetic Levitation Method. The technique was created.
Glauco Souza, a Brazilian researcher who works at Rice University (United States), together with some collaborators, and brought to IFSC by Luis Gustavo Sabino, a researcher from the Optics Group who visited the North American university, where he learned how to use the technique that allows tissue or tumor models to be obtained in vitro in approximately hours. Unlike conventional monolayer cultivation, in whi Whatsapp Number List ch cells adhere to cell culture bottles, three-dimensional cultivation techniques, such as levitation, allow for better prediction of in vivo events , as cell growth resembles that of natural tissue. “In the body, cancer cells also grow three-dimensionally. Thus, the action of a given compound, such as that of a photosensitizer, is influenced by its diffusion within a solid tumor, which interferes with the treatment of diseases such as melanoma”, he explains. In order for the malignant cells to behave as if they were in the human body, they were incubated together with ferromagnetic nanoparticles and.
Using a magnet (known as neodymium), they levitated, forming melanoma tumors. Then, the tumors were incubated with Photodithazine (photosensitizing molecule that was applied in a non-toxic concentration) and received PDT application. Photodynamic Therapy In in vitro tests , Larissa applied Photodynamic Therapy to tumors with different thicknesses. When she provided LED (light-emitting diode) illumination on an eighty micrometer tumor, the photosensitizing molecule was excited, causing % tumor death. When applying the treatment to a tumor with a slightly greater thickness (one hundred and thirty micrometers), this percentage dropped to %. “These results are promising, because not only did we observe a more homogeneous distribution of Photodithazine in melanoma tumors, to ensure that the treatment of the disease was not partial, but we also analyzed signs of great tumor breakdown and death”, says the IFSC researcher . Although this rate decreased by % during the application.
Glauco Souza, a Brazilian researcher who works at Rice University (United States), together with some collaborators, and brought to IFSC by Luis Gustavo Sabino, a researcher from the Optics Group who visited the North American university, where he learned how to use the technique that allows tissue or tumor models to be obtained in vitro in approximately hours. Unlike conventional monolayer cultivation, in whi Whatsapp Number List ch cells adhere to cell culture bottles, three-dimensional cultivation techniques, such as levitation, allow for better prediction of in vivo events , as cell growth resembles that of natural tissue. “In the body, cancer cells also grow three-dimensionally. Thus, the action of a given compound, such as that of a photosensitizer, is influenced by its diffusion within a solid tumor, which interferes with the treatment of diseases such as melanoma”, he explains. In order for the malignant cells to behave as if they were in the human body, they were incubated together with ferromagnetic nanoparticles and.
Using a magnet (known as neodymium), they levitated, forming melanoma tumors. Then, the tumors were incubated with Photodithazine (photosensitizing molecule that was applied in a non-toxic concentration) and received PDT application. Photodynamic Therapy In in vitro tests , Larissa applied Photodynamic Therapy to tumors with different thicknesses. When she provided LED (light-emitting diode) illumination on an eighty micrometer tumor, the photosensitizing molecule was excited, causing % tumor death. When applying the treatment to a tumor with a slightly greater thickness (one hundred and thirty micrometers), this percentage dropped to %. “These results are promising, because not only did we observe a more homogeneous distribution of Photodithazine in melanoma tumors, to ensure that the treatment of the disease was not partial, but we also analyzed signs of great tumor breakdown and death”, says the IFSC researcher . Although this rate decreased by % during the application.